SEC24A Recombinant monoclonal antibody

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  WB analysis using 82908-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82908-1-RR (SEC24A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82908-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC24A antibody (82908-1-RR, Clone: 230105B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 82908-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SEC24A (82908-1-RR, Clone:230105B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82908-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82908-1-RR against Human SEC24A were performed. The affinity constant is below 1 pM.

SEC24A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 82908-1-RR

Clone No.230105B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

230105B3, Protein transport protein Sec24A, SEC24-related protein A

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Tested Applications

• Positive WB detected in: U-87 MG cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82908-1-RR targets SEC24A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8723

  Product name: Recombinant human SEC24A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-613 aa of BC019341

  Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR

• Full Name: SEC24 family, member A (S. cerevisiae)
• Calculated Molecular Weight: 613 aa, 66 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC019341
• Gene Symbol: SEC24A
• Gene ID (NCBI): 10802
• RRID: AB_3670637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95486
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.