SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G5G9

Cat no : 67409-1-Ig

Synonyms

FLJ43974, KIAA0755, SEC24 related protein D, SEC24D

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Validation Data Gallery

• 

  WB analysis of Jurkat using 67409-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67409-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67409-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67409-1-Ig targets SEC24D in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC24D fusion protein Ag4657
• Full Name: SEC24 family, member D (S. cerevisiae)
• Calculated Molecular Weight: 1033 aa, 113 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC035761
• Gene Symbol: SEC24D
• Gene ID (NCBI): 9871
• RRID: AB_2882650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).