SEC61 Alpha Polyclonal antibody, PBS Only

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• SEC61 Alpha Polyclonal antibody, PBS Only (24935-1-PBS)

• 

  WB analysis of mouse brain using 24935-1-AP (same clone as 24935-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24935-1-AP (same clone as 24935-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 24935-1-AP (same clone as 24935-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61 Alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 24935-1-AP (same clone as 24935-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

SEC61 Alpha Polyclonal Antibody for WB, Indirect ELISA

Cat No. 24935-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

SEC61A2, Sec61 alpha-2, Protein transport protein Sec61 subunit alpha isoform 2

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Product Information

24935-1-PBS targets SEC61 Alpha in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20779

  Product name: Recombinant human SEC61A2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 190-418 aa of BC026179

  Sequence: TIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNSARA

• Full Name: Sec61 alpha 2 subunit (S. cerevisiae)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC026179
• Gene Symbol: SEC61 Alpha
• Gene ID (NCBI): 55176
• RRID: AB_2879807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9S3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).