SEL1L Recombinant monoclonal antibody

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• SEL1L Recombinant monoclonal antibody (84317-7-RR)

SEL1L Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84317-7-RR Copied!

Clone No. 241594G2

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

241594G2, Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L

• 

  WB analysis using 84317-7-RR

  Mouse kidney tissue, rat liver tissue were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 84317-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 84317-7-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84317-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, mouse kidney tissue, rat liver tissue
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag32178

  Product name: Recombinant human SEL1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-225 aa of NM_005065

  Sequence: SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTGMKILNGSNKKSQKREAYRYLQKAASMNHTKALERV

• Full Name: sel-1 suppressor of lin-12-like (C. elegans)
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: NM_005065
• Gene Symbol: SEL1L
• Gene ID (NCBI): 6400
• RRID: AB_3671858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBV2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .

Publications

What published studies show

Antibody 84317-7-RR (anti-TNFRSF11B/Osteoprotegerin, Rabbit monoclonal) has 1 citation to date. The paper, published in Bone (IF 3.9), investigates how disease-associated TNFRSF11B variants differentially disrupt osteoprotegerin secretion and function in mouse models. The antibody was applied in Western Blot (WB) with RNAi validation. The research falls within the fields of bone biology, osteoporosis, and receptor-mediated signaling.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Bone; Disease-associated TNFRSF11B variants differentially disrupt osteoprotegerin secretion and function.; Authors - Reina Suzuki ; KD Validated