SENP3 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85837-3-RR (same clone as 85837-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85837-3-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 85837-3-RR (same clone as 85837-3-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85837-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85837-3-RR (same clone as 85837-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SENP3 antibody (85837-3-RR, Clone: 250153A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85837-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02141-2

  Cytometric bead array standard curve of MP02141-2, SENP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85837-3-PBS. Detection antibody: 85837-2-PBS. Standard: Ag11911. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP02141-1

  Cytometric bead array standard curve of MP02141-1, SENP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85837-3-PBS. Detection antibody: 85837-1-PBS. Standard: Ag11911. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 85837-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85837-3-RR against Human SENP3 were performed. The affinity constant is 0.578 nM.

SENP3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 85837-3-PBS

Clone No.250153A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3

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Product Information

85837-3-PBS targets SENP3 as part of a matched antibody pair:

MP02141-1: 85837-3-PBS capture and 85837-1-PBS detection (validated in Cytometric bead array)

MP02141-2: 85837-3-PBS capture and 85837-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SENP3 fusion protein Ag11911
• Full Name: SUMO1/sentrin/SMT3 specific peptidase 3
• Calculated Molecular Weight: 574 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC080658
• Gene Symbol: SENP3
• Gene ID (NCBI): 26168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H4L4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.