SENP3 Recombinant antibody

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  WB analysis using 85837-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 85837-3-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 85837-3-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SENP3 antibody (85837-3-RR, Clone: 250153A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85837-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85837-3-RR against Human SENP3 were performed. The affinity constant is 0.578 nM.

SENP3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85837-3-RR

Clone No.250153A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3

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Tested Applications

• Positive WB detected in: Y79 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Caco-2 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85837-3-RR targets SENP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SENP3 fusion protein Ag11911
• Full Name: SUMO1/sentrin/SMT3 specific peptidase 3
• Calculated Molecular Weight: 574 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC080658
• Gene Symbol: SENP3
• Gene ID (NCBI): 26168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H4L4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.