SERPIND1 Monoclonal antibody

SERPIND1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2C1E1

Cat no : 67602-1-Ig

Synonyms

D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1

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Validation Data Gallery

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  WB analysis using 67602-1-Ig

  0.8 μL, 0.4 μL, and 0.2 μL human plasma lysates were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig plasma using 67602-1-Ig

  0.4 μL pig plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 67602-1-Ig

  0.2 μLhuman plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 67602-1-Ig

  0.4 μL human plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67602-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67602-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67602-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67602-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPIND1 antibody (67602-1-Ig, Clone: 2C1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma, human placenta tissue, pig plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67602-1-Ig targets SERPIND1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERPIND1 fusion protein Ag26351
• Full Name: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• Calculated Molecular Weight: 499 aa, 57 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC035028
• Gene Symbol: SERPIND1
• Gene ID (NCBI): 3053
• RRID: AB_2882808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.