SERPIND1 Monoclonal antibody, PBS Only

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  WB analysis using 67602-1-Ig (same clone as 67602-1-PBS)

  0.8 μL, 0.4 μL, and 0.2 μL human plasma lysates were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig plasma using 67602-1-Ig (same clone as 67602-1-PBS)

  0.4 μL pig plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 67602-1-Ig (same clone as 67602-1-PBS)

  0.2 μLhuman plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 67602-1-Ig (same clone as 67602-1-PBS)

  0.4 μL human plasma were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67602-1-Ig (same clone as 67602-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67602-1-Ig (same clone as 67602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67602-1-Ig (same clone as 67602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67602-1-Ig (same clone as 67602-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPIND1 antibody (67602-1-Ig, Clone: 2C1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67602-1-PBS in a different storage buffer formulation.

SERPIND1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67602-1-PBS

Clone No.2C1E1

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HC II, HCF2, HLS2, Serpin D1, 2C1E1

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Product Information

67602-1-PBS targets SERPIND1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26351

  Product name: Recombinant human SERPIND1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 20-300 aa of BC035028

  Sequence: GSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNIVNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWV

• Full Name: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• Calculated Molecular Weight: 499 aa, 57 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC035028
• Gene Symbol: SERPIND1
• Gene ID (NCBI): 3053
• RRID: AB_2882808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05546
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.