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  • KD/KO Validated

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11231-1-AP

Synonyms

ESET, H3 K9 HMTase 4, H3 K9 HMTase4, KG1T, KIAA0067, KMT1E, Lysine N methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1, SETDB1



Tested Applications

Positive WB detected inJurkat cells, PC-3 cells, mouse brain tissue, human testis tissue, MCF-7 cells, HEK-293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11231-1-AP targets SETDB1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETDB1 fusion protein Ag1725
Full Name SET domain, bifurcated 1
Calculated molecular weight 143 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC009362
Gene symbol SETDB1
Gene ID (NCBI) 9869
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

Protocols

Product Specific Protocols
WB protocol for SETDB1 antibody 11231-1-APDownload protocol
IHC protocol for SETDB1 antibody 11231-1-APDownload protocol
IP protocol for SETDB1 antibody 11231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions.

Authors - Jianping Guo

Oncol Rep

SETDB1 induces epithelial‑mesenchymal transition in breast carcinoma by directly binding with Snail promoter.

Authors - Wenlin Yang
humanIHC

Med Sci Monit

High SET Domain Bifurcated 1 (SETDB1) Expression Predicts Poor Prognosis in Breast Carcinoma.

Authors - Zhaoping Zhou
humanWB,chIP

Cell Death Dis

SETDB1 promotes the progression of colorectal cancer via epigenetically silencing p21 expression.

Authors - Nan Cao
  • KD Validated
mousechIP

Biochim Biophys Acta

Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway.

Authors - Tiantian Liu
mouseWB

Toxicol Appl Pharmacol

Melatonin protects mouse spermatogonial stem cells against hexavalent chromium-induced apoptosis and epigenetic histone modification.

Authors - Yinghua Lv