• Featured Product
  • KD/KO Validated

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11231-1-AP

Synonyms

ESET, H3 K9 HMTase 4, H3 K9 HMTase4, KG1T, KIAA0067, KMT1E, Lysine N methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1, SETDB1



Tested Applications

Positive WB detected inJurkat cells, PC-3 cells, human testis tissue, MCF-7 cells, HEK-293 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11231-1-AP targets SETDB1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETDB1 fusion protein Ag1725
Full Name SET domain, bifurcated 1
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
RRIDAB_2186069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

Protocols

Product Specific Protocols
WB protocol for SETDB1 antibody 11231-1-APDownload protocol
IHC protocol for SETDB1 antibody 11231-1-APDownload protocol
IF protocol for SETDB1 antibody 11231-1-APDownload protocol
IP protocol for SETDB1 antibody 11231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
mouseWB,CoIP

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
mouse,humanWB

Nature

Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.

Authors - Gabriel K Griffin
  • KO Validated
mouseWB,CoIP,ChIP

Nature

KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements

Authors - Shang-Min Zhang
humanWB

Nature

Genome surveillance by HUSH-mediated silencing of intronless mobile elements.

Authors - Marta Seczynska
  • KD Validated
humanWB

Science

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Authors - Iva A Tchasovnikarova
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Taku (Verified Customer) (06-15-2022)

  • Applications: Western Blot
  • Primary Antibody Dilution: x2000
  • Cell Tissue Type: T cell