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SFXN1 Polyclonal antibody, PBS Only (12296-1-PBS)

Filter: AllWBIPIHC

WB analysis using 12296-1-AP (same clone as 12296-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 12296-1-AP (same clone as 12296-1-PBS)
WB result of SFXN1 antibody (12296-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 12296-1-AP (same clone as 12296-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12296-1-AP (same clone as 12296-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12296-1-AP (same clone as 12296-1-PBS)
IP result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SFXN1 Polyclonal antibody, PBS Only

Name: SFXN1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12296-1-PBS

SFXN1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12296-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

12296-1-PBS targets SFXN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2985 Product name: Recombinant human SFXN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC020517 Sequence: MILIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAVVNYTNRSGDAPLTVNELGTAYVSATTGAVATALGLNALTKHVSPLIGRFVPFAAVAAANCINIPLMRQRELKVGIPVTDENGNRLGESANAAKQAITQVVVSRILMAAPGMAIPPFIMNTLEKKAFLKRFPWMSAPIQVGLVGFCLVFATPLCCALFPQKSSMSVTSLEAELQAKIQESHPELRRVYFNKGL Predict reactive species
Full Name	sideroflexin 1
Calculated Molecular Weight	35.6 kDa
Observed Molecular Weight	30-35 kDa
GenBank Accession Number	BC020517
Gene Symbol	SFXN1
Gene ID (NCBI)	94081
RRID	AB_2185814
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H9B4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.