SFXN3 Recombinant antibody

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  WB analysis using 85978-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85978-1-RR (SFXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of C6 using 85978-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using SFXN3 antibody (85978-1-RR, Clone: 250485B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SFXN3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85978-1-RR

Clone No.250485B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SFX3, sideroflexin 3, Sideroflexin-3

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Tested Applications

• Positive WB detected in: Recombinant protein, BxPC-3 cells, NIH/3T3 cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85978-1-RR targets SFXN3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SFXN3 fusion protein Ag7203
• Full Name: sideroflexin 3
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000124
• Gene Symbol: SFXN3
• Gene ID (NCBI): 81855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BWM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.