SH2D2A Polyclonal antibody

SH2D2A Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18539-1-AP

Synonyms

T cell-specific adapter protein, SH2 domain-containing protein 2A, SH2 domain-containing adapter protein, SH2 domain protein 2A, SCAP

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Validation Data Gallery

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  WB analysis of K-562 using 18539-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 18539-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18539-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of K-562 using 18539-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug SH2D2A Polyclonal antibody (18539-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18539-1-AP targets SH2D2A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH2D2A fusion protein Ag13355
• Full Name: SH2 domain protein 2A
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC012107
• Gene Symbol: SH2D2A
• Gene ID (NCBI): 9047
• RRID: AB_10640158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mult Scler Relat Disord; Downregulation of lncRNA XIST may promote Th17 differentiation through KDM6A-TSAd pathway in neuromyelitis optica spectrum disorders; Authors - Ruo-Yi Guo