Sur-8 Polyclonal antibody

Sur-8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17561-1-AP

Synonyms

KIAA0862, Protein soc 2 homolog, Protein sur 8 homolog, SHOC2, SIAA0862, SOC 2, SOC2, SUR 8, SUR8, sur-8



Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, mouse brain tissue, rat testis tissue, U-251 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17561-1-AP targets Sur-8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sur-8 fusion protein Ag11754
Full Name soc-2 suppressor of clear homolog (C. elegans)
Calculated molecular weight 582 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC050445
Gene symbol SHOC2
Gene ID (NCBI) 8036
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Sur-8 antibody 17561-1-APDownload protocol
IHC protocol for Sur-8 antibody 17561-1-APDownload protocol
IF protocol for Sur-8 antibody 17561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Biol Cell

VCP/p97 controls signals of the ERK1/2 pathway transmitted via the Shoc2 scaffolding complex: novel insights into IBMPFD pathology.

Authors - HyeIn Jang
humanWB

Cell Signal

Shoc2-tranduced ERK1/2 motility signals - Novel insights from functional genomics.

Authors - Myoungkun Jeoung
humanWB, IP

Mol Cell Biol

HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold.

Authors - Eun Ryoung Jang
monkeyWB, IP

J Cell Sci

Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.

Authors - Eun Ryoung Jang
mouseWB

EMBO J

Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis.

Authors - Philbert Lee
humanIP,WB

J Clin Invest

Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation.

Authors - Harmon Robert M RM