SIRP Beta 1 Monoclonal antibody

SIRP Beta 1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A3H1

Cat no : 68206-1-Ig

Synonyms

CD172b, DKFZp686A05192, FLJ26614, SIRP BETA 1, SIRPB1

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Validation Data Gallery

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  WB analysis using 68206-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human lung cancer using 68206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat cerebellum tissue, rabbit cerebellum tissue, pig cerebellum tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, pig brain tissue, U-937 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68206-1-Ig targets SIRP Beta 1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRP Beta 1 fusion protein Ag28615
• Full Name: signal-regulatory protein beta 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC025286
• Gene Symbol: SIRPB1
• Gene ID (NCBI): 10326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.