SIRT7 Polyclonal antibody, PBS Only

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• SIRT7 Polyclonal antibody, PBS Only (12994-1-PBS)

• 

  WB analysis using 12994-1-AP (same clone as 12994-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 12994-1-AP (same clone as 12994-1-PBS)

  IP result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:1000) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SIRT7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12994-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

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Product Information

12994-1-PBS targets SIRT7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3656

  Product name: Recombinant human SIRT7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC017305

  Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

• Full Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Calculated Molecular Weight: 400 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017305
• Gene Symbol: SIRT7
• Gene ID (NCBI): 51547
• RRID: AB_10644276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRC8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.