SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1


human, pig, rat, mouse







Cat no : 66367-1-Ig


PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

Tested Applications

Positive WB detected inRAW 264.7 cells, human spleen tissue, pig liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66367-1-Ig targets SKAP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Reactivity human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SKAP2 fusion protein Ag3615
Full Name src kinase associated phosphoprotein 2
Calculated molecular weight 41 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC036044
Gene symbol SKAP2
Gene ID (NCBI) 8935
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.


Product Specific Protocols
WB protocol for SKAP2 antibody 66367-1-IgDownload protocol
IHC protocol for SKAP2 antibody 66367-1-IgDownload protocol
IF protocol for SKAP2 antibody 66367-1-IgDownload protocol
Standard Protocols
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