SKIV2L2 Polyclonal antibody, PBS Only

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• SKIV2L2 Polyclonal antibody, PBS Only (12719-2-PBS)

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  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12719-2-AP (same clone as 12719-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 12719-2-AP (same clone as 12719-2-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 12719-2-AP (same clone as 12719-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 12719-2-AP (same clone as 12719-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SKIV2L2 antibody (12719-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 12719-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

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Product Information

12719-2-PBS targets SKIV2L2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3414

  Product name: Recombinant human SKIV2L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3-307 aa of BC028604

  Sequence: DAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEGTDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFILDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVI

• Full Name: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 1042 aa, 118 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC028604
• Gene Symbol: SKIV2L2
• Gene ID (NCBI): 23517
• RRID: AB_2187479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42285
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.