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  • KD/KO Validated

SKP2 Polyclonal antibody

SKP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 15010-1-AP


F box protein Skp2, F box/LRR repeat protein 1, FBL1, FBXL1, FLB1, p45skp2, SKP2

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15010-1-AP targets SKP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKP2 fusion protein Ag6826
Full Name S-phase kinase-associated protein 2 (p45)
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC001441
Gene symbol SKP2
Gene ID (NCBI) 6502
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for SKP2 antibody 15010-1-APDownload protocol
IHC protocol for SKP2 antibody 15010-1-APDownload protocol
IF protocol for SKP2 antibody 15010-1-APDownload protocol
IP protocol for SKP2 antibody 15010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Cell

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

Authors - Shihao Zhang

Nat Commun

DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.

Authors - Alexis R Barr

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H


TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Authors - Kailiang Zhou

Cell Rep

Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.

Authors - Alison A Lanctot

J Med Chem

Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.

Authors - Jin Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jonathan (Verified Customer) (01-11-2022)

Good clear signal with no background issues. I'm happy with the blots obtained using this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HeLa