|Positive WB detected in||HEK-293 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15010-1-AP targets SKP2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SKP2 fusion protein Ag6826|
|Full Name||S-phase kinase-associated protein 2 (p45)|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC001441|
|Gene ID (NCBI)||6502|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for SKP2 antibody 15010-1-AP||Download protocol|
|IHC protocol for SKP2 antibody 15010-1-AP||Download protocol|
|IF protocol for SKP2 antibody 15010-1-AP||Download protocol|
|IP protocol for SKP2 antibody 15010-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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