• Featured Product
  • KD/KO Validated

SKP2 Polyclonal antibody

SKP2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15010-1-AP

Synonyms

F box protein Skp2, F box/LRR repeat protein 1, FBL1, FBXL1, FLB1, p45skp2, SKP2



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15010-1-AP targets SKP2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKP2 fusion protein Ag6826
Full Name S-phase kinase-associated protein 2 (p45)
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC001441
Gene symbol SKP2
Gene ID (NCBI) 6502
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SKP2 antibody 15010-1-APDownload protocol
IHC protocol for SKP2 antibody 15010-1-APDownload protocol
IF protocol for SKP2 antibody 15010-1-APDownload protocol
IP protocol for SKP2 antibody 15010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Physiol Biochem

PMID: 29258063

Authors - Linting Wei
ratWB

Cell Death Dis

FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.

Authors - Kailiang Zhou
human,mouseIHC

J Clin Med

The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy.

Authors - Yi-Chun Tsai
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
mouseWB

Am J Physiol Renal Physiol

MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.

Authors - Chen Ye
humanIHC, WB

Cell Death Dis

p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response.

Authors - Chuang Li