SKP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SKP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (1)

Cat no : 15010-1-AP

Synonyms

F box protein Skp2, F box/LRR repeat protein 1, FBL1, FBXL1, FLB1, p45skp2, SKP2

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Validation Data Gallery

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  WB analysis of HEK-293 using 15010-1-AP

  WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells.

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  WB analysis using 15010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15010-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15010-1-AP

  WB result of SKP2 antibody (15010-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 15010-1-AP

  IP Result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug.

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  IHC staining of human lung cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15010-1-AP

  Immunofluorescent analysis of HepG2 cells using 15010-1-AP (SKP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 34 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

15010-1-AP targets SKP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKP2 fusion protein Ag6826
• Full Name: S-phase kinase-associated protein 2 (p45)
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC001441
• Gene symbol: SKP2
• Gene ID (NCBI): 6502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Cell; Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.; Authors - Shihao Zhang
human	WB	Nat Commun; DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.; Authors - Alexis R Barr
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
mouse	IF,IP	Cell Rep; Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.; Authors - Alison A Lanctot
mouse	WB	J Med Chem; Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.; Authors - Jin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonathan (Verified Customer) (01-11-2022)

Good clear signal with no background issues. I'm happy with the blots obtained using this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HeLa