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SKP2 Polyclonal antibody, PBS Only (15010-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 15010-1-AP (same clone as 15010-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)
WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 15010-1-AP (same clone as 15010-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)
WB result of SKP2 antibody (15010-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)
IP result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 15010-1-AP (same clone as 15010-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (15010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SKP2 Polyclonal antibody, PBS Only

Name: SKP2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15010-1-PBS

SKP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15010-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15010-1-PBS targets SKP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6826 Product name: Recombinant human SKP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC001441 Sequence: MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSR Predict reactive species
Full Name	S-phase kinase-associated protein 2 (p45)
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	46-48 kDa
GenBank Accession Number	BC001441
Gene Symbol	SKP2
Gene ID (NCBI)	6502
RRID	AB_2187647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13309
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).