MCT2/SLC16A7 Recombinant antibody

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  WB analysis using 85145-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85145-3-RR (SLC16A7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85145-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85145-3-RR against Human MCT2/SLC16A7 were performed. The affinity constant is 37.0 nM.

MCT2/SLC16A7 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85145-3-RR

Clone No.242811F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SLC16A7, MCT 2, MCT2, Monocarboxylate transporter 2, Solute carrier family 16 member 7

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Tested Applications

• Positive WB detected in: BxPC-3 cells, K-562 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85145-3-RR targets MCT2/SLC16A7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MCT2/SLC16A7 fusion protein Ag14201
• Full Name: solute carrier family 16, member 7 (monocarboxylic acid transporter 2)
• Calculated Molecular Weight: 478 aa, 52 kDa
• Observed Molecular Weight: 40-52 kDa
• GenBank Accession Number: BC030693
• Gene Symbol: MCT2
• Gene ID (NCBI): 9194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60669
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC16A7, also named MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in the brain, cerebellum, liver, and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung, or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. The 80 kDa protein might be a dimer, similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736)