SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F11A3

Cat no : 66771-1-Ig

Synonyms

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

---

Validation Data Gallery

• 

  WB analysis using 66771-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66771-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66771-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HST-C6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

66771-1-Ig targets SLC25A1 in WB, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC25A1 fusion protein Ag7520
• Full Name: solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC004980
• Gene symbol: SLC25A1
• Gene ID (NCBI): 6576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.; Authors - Weiwei Liu