SLC25A22 Recombinant antibody

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  WB analysis using 86163-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86163-1-RR (SLC25A22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SLC25A22 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86163-1-RR

Clone No.250788B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

GC 1, GC1, GC-1, Glutamate/H(+) symporter 1, NET44

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86163-1-RR targets SLC25A22 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC25A22 fusion protein Ag19958
• Full Name: solute carrier family 25 (mitochondrial carrier: glutamate), member 22
• Calculated Molecular Weight: 323 aa, 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC023545
• Gene Symbol: SLC25A22
• Gene ID (NCBI): 79751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H936
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)