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SLC25A24 Recombinant monoclonal antibody, PBS Only (Detector) (86635-1-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISAIHC

WB analysis using 86635-1-RR (same clone as 86635-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86635-1-RR (SLC25A24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86635-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02724-1
The mean SLC25A24 concentration was determined to be 3,148.64 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load and 3,459.34 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load.
Sandwich ELISA standard curve of MP02724-1
Sandwich ELISA standard curve of MP02724-1, Human SLC25A24 Recombinant Matched Antibody Pair - PBS only. 86635-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6334. 86635-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL.
IHC staining of Human Hepatocellular cancer using 86635-1-RR (same clone as 86635-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 86635-1-RR (SLC25A24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86635-1-PBS in a different storage buffer formulation.

SLC25A24 Recombinant monoclonal antibody, PBS Only (Detector)

Name: SLC25A24 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 86635-1-PBS

SLC25A24 Uni-rAb^® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Cat No. 86635-1-PBS

Clone No.251563C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Synonyms

APC1, MCSC1, Mitochondrial adenyl nucleotide antiporter SLC25A24, Mitochondrial ATP-Mg/Pi carrier protein 1, Mitochondrial Ca(2+)-dependent solute carrier protein 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

86635-1-PBS targets SLC25A24 as part of a matched antibody pair:

MP02724-1: 86635-2-PBS capture and 86635-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6334 Product name: Recombinant human SLC25A24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-477 aa of BC068561 Sequence: ELILQSIDVDGTMTVDWNEWRDYFLFNPVTDIEEIIRFWKHSTGIDIGDSLTIPDEFTEDEKKSGQWWRQLLAGGIAGAVSRTSTAPLDRLKIMMQVHGSKSDKMNIFGGFRQMVKEGGIRSLWRGNGTNVIKIAPETAVKFWAYEQYKKLLTEEGQKIGTFERFISGSMAGATAQTFIYPMEVMKTRLAVGKTGQYSGIYDCAKKILKHEGLGAFYKGYVPNLLGIIPYAGIDLAVYELLKSYWLDNFAKDSVNPGVMVLLGCGALSSTCGQLASYPLALVRTRMQAQAMLEGSPQLNMVGLFRRIISKEGIPGLYRGITPNFMKVLPAVGISYVVYENMKQTLGVTQK Predict reactive species
Full Name	solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC068561
Gene Symbol	SLC25A24
Gene ID (NCBI)	29957
RRID	AB_3745021
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6NUK1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SLC25A24 (solute carrier family 25 member 24) is a gene encoding a mitochondrial ATP-Mg/phosphate transporter. It belongs to the mitochondrial carrier protein family and is mainly involved in mitochondrial energy metabolism and calcium signal regulation.