SLC25A31/ANT4 Polyclonal antibody, PBS Only

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• SLC25A31/ANT4 Polyclonal antibody, PBS Only (33263-1-PBS)

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  WB analysis using 33263-1-AP (same clone as 33263-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33263-1-AP (ANT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue, mouse heart tissue. This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 33263-1-AP (same clone as 33263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 33263-1-AP (same clone as 33263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 33263-1-AP (same clone as 33263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 33263-1-AP (same clone as 33263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 33263-1-AP (same clone as 33263-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SLC25A31/ANT4 antibody (33263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

SLC25A31/ANT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 33263-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ANT4, SLC25A31, AAC4, Adenine nucleotide translocator 4, ADP,ATP carrier protein 4

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Product Information

33263-1-PBS targets SLC25A31/ANT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11008

  Product name: Recombinant human SLC25A31 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC022032

  Sequence: MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR

• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC022032
• Gene Symbol: SLC25A31
• Gene ID (NCBI): 83447
• RRID: AB_3742919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H0C2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLC25A31, also known as ANT4, is a member of the mitochondrial carrier family. The proteins of this family are mainly involved in the transport of substances inside and outside the mitochondria. SLC25A31 is an ADP/ATP antiporter protein, which catalyzes the exchange of ATP generated in mitochondria by ATP synthase against ADP produced in cytosol by most energy-consuming reactions(PMID: 15670820). SLC25A31 is mainly expressed in the testis and male germ cells (PMID: 25031318, 23506884).