SLTM Polyclonal antibody, PBS Only

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• SLTM Polyclonal antibody, PBS Only (17889-1-PBS)

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  WB analysis of mouse brain using 17889-1-AP (same clone as 17889-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17889-1-AP (same clone as 17889-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17889-1-AP (same clone as 17889-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 17889-1-AP (same clone as 17889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17889-1-AP (same clone as 17889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLTM antibody (17889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

SLTM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

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Product Information

17889-1-PBS targets SLTM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12060

  Product name: Recombinant human SLTM protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 154-502 aa of BC046119

  Sequence: RERIRIEQERRKEAERIAREREELRRQQQQLRYEQEKRNSLKRPRDVDHRRDDPYWSENKKLSLDTDARFGHGSDYSRQQNRFNDFDHRERGRFPESSAVQSSSFERRDRFVGQSEGKKARPTARREDPSFERYPKNFSDSRRNEPPPPRNELRESDRREVRGERDERRTVIIHDRPDITHPRHPREAGPNPSRPTSWKSEGSMSTDKRETRVERPERSGREVSGHSVRGAPPGNRSSASGYGSREGDRGVITDRGGGSQHYPEERHVVERHGRDTSGPRKEWHGPPSQGPSYHDTRRMGDGRAGAGMITQHSSNASPINRIVQISGNSMPRGSGSGFKPFKGGPPRRF

• Full Name: SAFB-like, transcription modulator
• Calculated Molecular Weight: 478aa,57 kDa; 1034aa,117 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC046119
• Gene Symbol: SLTM
• Gene ID (NCBI): 79811
• RRID: AB_2192729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NWH9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.