SMCR7L Monoclonal antibody

SMCR7L Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3B3G3

Cat no : 67808-1-Ig

Synonyms

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67808-1-Ig targets SMCR7L in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMCR7L fusion protein Ag13775
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Protocols

Product Specific Protocols
WB protocol for SMCR7L antibody 67808-1-IgDownload protocol
IHC protocol for SMCR7L antibody 67808-1-IgDownload protocol
IF protocol for SMCR7L antibody 67808-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols