SMCR7L Monoclonal antibody
SMCR7L Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse , rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3B3G3
Cat no : 67808-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
67808-1-Ig targets SMCR7L in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse , rat samples.
Tested Reactivity | Human, mouse , rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | SMCR7L fusion protein Ag13775 |
Full Name | Smith-Magenis syndrome chromosome region, candidate 7-like |
Calculated molecular weight | 463 aa, 51 kDa |
Observed molecular weight | 48-51 kDa |
GenBank accession number | BC002587 |
Gene symbol | SMCR7L |
Gene ID (NCBI) | 54471 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.
Protocols
Product Specific Protocols | |
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WB protocol for SMCR7L antibody 67808-1-Ig | Download protocol |
IHC protocol for SMCR7L antibody 67808-1-Ig | Download protocol |
IF protocol for SMCR7L antibody 67808-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |