SMURF1 Polyclonal antibody, PBS Only

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• SMURF1 Polyclonal antibody, PBS Only (55175-1-PBS)

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  WB analysis using 55175-1-AP (same clone as 55175-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55175-1-AP (SMURF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 55175-1-AP (same clone as 55175-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 55175-1-AP (same clone as 55175-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SMURF1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 55175-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625

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Product Information

55175-1-PBS targets SMURF1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SMAD specific E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_020429
• Gene Symbol: SMURF1
• Gene ID (NCBI): 57154
• RRID: AB_10859079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCE7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).