SNRPA1 Recombinant antibody

• 

  WB analysis using 85649-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85649-2-RR (SNRPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 85649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 85649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNRPA1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85649-2-RR

Clone No.243097D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

U2 small nuclear ribonucleoprotein A', U2 snRNP A, U2 snRNP A'

---

Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, Raji cells, HeLa cells, mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85649-2-RR targets SNRPA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNRPA1 fusion protein Ag10790
• Full Name: small nuclear ribonucleoprotein polypeptide A'
• Calculated Molecular Weight: 255 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC022816
• Gene Symbol: SNRPA1
• Gene ID (NCBI): 6627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09661
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.