Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, A549 cells, K-562 cells |
| Positive IHC detected in | human pancreas cancer tissue, rat kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68094-1-Ig targets SPTLC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28380 Product name: Recombinant human SPTLC2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 254-562 aa of BC005123 Sequence: CLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED Predict reactive species |
| Full Name | serine palmitoyltransferase, long chain base subunit 2 |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC005123 |
| Gene Symbol | SPTLC2 |
| Gene ID (NCBI) | 9517 |
| RRID | AB_2918831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPTLC2 antibody 68094-1-Ig | Download protocol |
| WB protocol for SPTLC2 antibody 68094-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPTLC2 Monoclonal antibody (2G6A12), catalog number 68094-1-Ig, has 1 citation. It appears in Frontiers in Cell and Developmental Biology, in a study on measles virus reprogramming of CD4⁺ T-cell sphingolipid and fatty acid metabolism to facilitate infection. The associated research fields include virology, T-cell immunology, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Measles virus reprograms CD4(+) T-cell sphingolipid and fatty acid metabolism to facilitate infection. |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The user tested it for Western Blot on a fibroblast cell line at a 1:2000 dilution and reported that it consistently delivers reliable results, describing it as a "very good antibody." Overall, the feedback highlights its performance and reproducibility in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nancy (Verified Customer) (01-29-2026) | Very good antibody. It always gives me consistent results.
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