SPTLC2 Monoclonal antibody, PBS Only

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  WB analysis using 68094-1-Ig (same clone as 68094-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68094-1-Ig (SPTLC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68094-1-Ig (same clone as 68094-1-PBS)

  WB result of SPTLC2 antibody (68094-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SPTLC2 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68094-1-PBS

Clone No.2G6A12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2

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Product Information

68094-1-PBS targets SPTLC2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28380

  Product name: Recombinant human SPTLC2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 254-562 aa of BC005123

  Sequence: CLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED

• Full Name: serine palmitoyltransferase, long chain base subunit 2
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005123
• Gene Symbol: SPTLC2
• Gene ID (NCBI): 9517
• RRID: AB_2918831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15270
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).