STAT5B Monoclonal antibody

STAT5B Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D9B11

Cat no : 66427-1-Ig

Synonyms

STAT5, STAT5B

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Validation Data Gallery

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  WB analysis of Jurkat using 66427-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66427-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66427-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 66427-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66427-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human STAT5B (66427-1-Ig, Clone:1D9B11) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2a (KFA042), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66427-1-Ig targets STAT5B in WB, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT5B fusion protein Ag2709
• Full Name: signal transducer and activator of transcription 5B
• Calculated Molecular Weight: 787 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC020868
• Gene Symbol: STAT5B
• Gene ID (NCBI): 6777
• RRID: AB_2881798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).