STBD1 Monoclonal antibody

STBD1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A2G2

Cat no : 67018-1-Ig

Synonyms

FLJ41801, Genethonin 1, GENEX3414, GENX 3414, starch binding domain 1, STBD1

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Validation Data Gallery

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  WB analysis using 67018-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67018-1-Ig (STBD1 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of human liver cancer using 67018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

67018-1-Ig targets STBD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STBD1 fusion protein Ag28613
• Full Name: starch binding domain 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC022301
• Gene Symbol: STBD1
• Gene ID (NCBI): 8987
• RRID: AB_2882334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

Publications

Species	Application	Title
human	WB,IF,IP	J Clin Invest; Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance; Authors - Nannan Zhou
human	IHC	Nat Commun; Model-based analysis uncovers mutations altering autophagy selectivity in human cancer.; Authors - Zhu Han