STIL Monoclonal antibody

• Featured Product
• KD/KO Validated

STIL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E12C1

Cat no : 66876-1-Ig

Synonyms

TAL-1-interrupting locus protein, SIL, SCL-interrupting locus protein, 1E12C1

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Validation Data Gallery

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  WB analysis using 66876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66876-1-Ig (STIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 66876-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  IF Staining of A431 using 66876-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, HL-60 cells, Daudi cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66876-1-Ig targets STIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STIL fusion protein Ag19124
• Full Name: SCL/TAL1 interrupting locus
• Calculated Molecular Weight: 1287 aa, 143 kDa
• Observed Molecular Weight: 150-155 kDa
• GenBank Accession Number: BC126223
• Gene Symbol: STIL
• Gene ID (NCBI): 6491
• RRID: AB_2882210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Int; ncRNAs-mediated overexpression of STIL predict unfavorable prognosis and correlated with the efficacy of immunotherapy of hepatocellular carcinoma; Authors - Longwen Xu
human	WB	Cancer Cell Int; The biological function and clinical significance of STIL in osteosarcoma.; Authors - Shu-Fan Ji ; KD Validated
human	WB	J Cell Physiol; Primary cilium participates in radiation-induced bystander effects through TGF-β1 signaling; Authors - Pei Qu ; KD Validated
human	IF	Cell Rep; Dual roles of CCDC102A in governing centrosome duplication and cohesion; Authors - Tianning Wang