STK25 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86216-1-RR (same clone as 86216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86216-1-RR (STK25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86216-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02398-1

  Sandwich ELISA standard curve of MP02398-1, Human STK25 Recombinant Matched Antibody Pair - PBS only. 86216-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag22964. 86216-1-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

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  Sample test of MP02398-1

  The mean SNAI2/SLUG concentration was determined to be 4,108.0 pg/mL in LnCap cell extract based on a 2.0 mg/mL extract load and 3,191.3 pg/mL in U2OS cell extract based on a 2.2 mg/mL extract load.

STK25 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86216-1-PBS

Clone No.250202A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EC:2.7.11.1, Serine/threonine-protein kinase 25, SOK 1, SOK1, SOK-1

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Product Information

86216-1-PBS targets STK25 as part of a matched antibody pair:

MP02398-1: 86216-2-PBS capture and 86216-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STK25 fusion protein Ag22964
• Full Name: serine/threonine kinase 25 (STE20 homolog, yeast)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015793
• Gene Symbol: STK25
• Gene ID (NCBI): 10494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00506
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.