CoraLite® Plus 488-conjugated STK3/MST2 Monoclonal antibody

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  IF Staining of NIH/3T3 using CL488-66637

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 STK3 antibody (CL488-66637, Clone: 1C11A4 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL488-66637

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK3 (CL488-66637, Clone:1C11A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

STK3/MST2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66637

Clone No.1C11A4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

MST2, STK3, 1C11A4, EC:2.7.11.1, KRS1

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66637 targets STK3/MST2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2741

  Product name: Recombinant human STK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-306 aa of BC010640

  Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ

• Full Name: serine/threonine kinase 3 (STE20 homolog, yeast)
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC010640
• Gene Symbol: STK3
• Gene ID (NCBI): 6788
• RRID: AB_2934484
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13188
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The protein encoded by STK3 gene is a key serine/threonine kinase, and its protein product can be cleaved by caspase in the process of apoptosis, and the inhibitory C-terminal part is removed, and the N-terminal part is transferred to the nucleus to form homodimer, which becomes an active kinase and promotes chromatin condensation in the process of apoptosis. STK3 is one of the core components of Hippo signaling pathway, which plays a key role in controlling cell proliferation, apoptosis and tissue growth. In the Hippo signaling pathway, STK3 forms a cascade reaction with STK4, LATS1/2 and other kinases, phosphorylates and activates LATS1/2, and then inhibits transcription coactivators YAP and TAZ, thus controlling cell growth and preventing cancer progression. STK3 and STK4(MST1) are similar in structure and function. They have conserved amino-terminal kinase domains, carboxyl-terminal regulatory domains and interaction domains. The antibody can cross-recognize STK4.