CoraLite®594-conjugated STOML2 Monoclonal antibody

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  IF Staining of A431 using CL594-60052

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 STOML2 antibody (CL594-60052, Clone: 1A2E9 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

STOML2 Monoclonal Antibody for IF/ICC

Cat No. CL594-60052

Clone No.1A2E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

1A2E9, EPB72 like protein 2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60052 targets STOML2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0363

  Product name: Recombinant human STOML2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 82-356 aa of BC002442

  Sequence: IVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPVPGTPDSLSSGSSRDVQGTDASLDEELDRVKMS

• Full Name: stomatin (EPB72)-like 2
• Calculated Molecular Weight: 356 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC002442
• Gene Symbol: STOML2
• Gene ID (NCBI): 30968
• RRID: AB_3673433
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJZ1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.