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FC experiment of Human Bone marrow mesenchymal stromal cells using Biotin-FcA65585
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were then stained with FcZero-rAb® APC Anti-Human CD90 Rabbit Recombinant Antibody (APC-FcA98126, Clone: 241290B2). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were then stained with FcZero-rAb® APC Anti-Human CD90 Rabbit Recombinant Antibody (APC-FcA98126, Clone: 241290B2). Cells were not fixed.
FC experiment of Human Bone marrow mesenchymal stromal cells using Biotin-FcA65585
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.
FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-FcA65585 targets STRO-1 in FC applications and shows reactivity with human samples.
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
FC Figures
FC experiment of Human Bone marrow mesenchymal stromal cells using Biotin-FcA65585
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were then stained with FcZero-rAb® APC Anti-Human CD90 Rabbit Recombinant Antibody (APC-FcA98126, Clone: 241290B2). Cells were not fixed.
FC experiment of Human Bone marrow mesenchymal stromal cells using Biotin-FcA65585
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Biotin Anti-Human STRO-1 (STRO-1) Rabbit RecAb (Biotin-FcA65585, Clone: STRO-1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
STRO-1 Recombinant antibody
Catalog Number
Biotin-FcA65585
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet