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What's the best dilution for STRO-1 in ?
Tested Applications
| Positive FC detected in | Human Bone marrow mesenchymal stromal cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-FcA65585 targets STRO-1 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Human CD34 positive bone marrow cells Predict reactive species |
| Full Name | STRO-1 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
STRO-1, first identified in 1991, is a well-known cell surface marker to identify and isolate mesenchymal stem cells (MSCs), also known as mesenchymal precursor cells (MPCs). STRO-1 antigen is present on fibroblast colony-forming unit (CFU-F) cells in adult human BM and potentially defines a MSC precursor subpopulation. Western blot analysis identifies Stro-1 as a 75kd protein in endothelial cells (PMID: 21903091, 2070060).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 STRO-1 antibody CL555-FcA65585 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
