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SULF2 Polyclonal antibody, PBS Only (12260-1-PBS)

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WB analysis of mouse liver using 12260-1-AP (same clone as 12260-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
WB analysis using 12260-1-AP (same clone as 12260-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 12260-1-AP (same clone as 12260-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 12260-1-AP (same clone as 12260-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 12260-1-AP (same clone as 12260-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SULF2 Polyclonal antibody, PBS Only

Name: SULF2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12260-1-PBS

SULF2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12260-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Arylsulfatase, EC:3.1.6.1, EC:3.1.6.14, Extracellular sulfatase Sulf 2, Extracellular sulfatase Sulf-2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12260-1-PBS targets SULF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2915 Product name: Recombinant human SULF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 722-870 aa of BC020962 Sequence: MPGLTCFTHDNQHWQTAPFWTLGPFCACTSANNNTYWCMRTINETHNFLFCEFATGFLEYFDLNTDPYQLMNAVNTLDRDVLNQLHVQLMELRSCKGYKQCNPRTRNMDLGLKDGGSYEQYRQFQRRKWPEMKRPSSKSLGQLWEGWEG Predict reactive species
Full Name	sulfatase 2
Calculated Molecular Weight	870 aa, 96 kDa
Observed Molecular Weight	50 kDa, 130-150 kDa
GenBank Accession Number	BC020962
Gene Symbol	SULF2
Gene ID (NCBI)	55959
RRID	AB_11183762
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IWU5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SULF2, also named as KIAA1247, belongs to the sulfatase family. It exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. SULF2 can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. SULF2 has several forms: the unglycosylated 100 kDa SULF2, the glycosylated 120-150 kDa SULF2, the cleaved 72-75 kDa and 50 kDa SULF2.