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SULT1A3 Polyclonal antibody, PBS Only (19499-1-PBS)

Filter: AllWBIPIHC

WB analysis using 19499-1-AP (same clone as 19499-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of Caco-2 using 19499-1-AP (same clone as 19499-1-PBS)
Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19499-1-AP (same clone as 19499-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 19499-1-AP (same clone as 19499-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 19499-1-AP (same clone as 19499-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 19499-1-AP (same clone as 19499-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IP experiment of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)
IP result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.

SULT1A3 Polyclonal antibody, PBS Only

Name: SULT1A3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 19499-1-PBS

SULT1A3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 19499-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Sulfotransferase 1A3/1A4, SULT1 A3, SULT1A 3, SULT1A4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

19499-1-PBS targets SULT1A3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	30-34 kDa
GenBank Accession Number	NM_177552
Gene Symbol	SULT1A3
Gene ID (NCBI)	6818
RRID	AB_10642560
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P0DMM9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.