SUPV3L1 Recombinant monoclonal antibody

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• SUPV3L1 Recombinant monoclonal antibody (86706-1-RR)

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  WB analysis using 86706-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 86706-1-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 86706-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 86706-1-RR (SUPV3L1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86706-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPV3L1 antibody (86706-1-RR, Clone: 250855G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SUPV3L1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86706-1-RR

Clone No.250855G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1

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Tested Applications

• Positive WB detected in: HeLa cells, rat kidney tissue, HEK-293 cells, PC-3 cells, NIH/3T3 cells, 3T3-L1 cells, mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86706-1-RR targets SUPV3L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3542

  Product name: Recombinant human SUPV3L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 441-786 aa of BC036112

  Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD

• Full Name: suppressor of var1, 3-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 786 aa, 88 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC036112
• Gene Symbol: SUPV3L1
• Gene ID (NCBI): 6832
• RRID: AB_3745068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IYB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).