CoraLite® Plus 488-conjugated SUPV3L1 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated SUPV3L1 Recombinant monoclonal antibody (CL488-86706)

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  IF Staining of HeLa using CL488-86706

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SUPV3L1 antibody (CL488-86706, Clone: 250855G8 ) at dilution of 1:200, CL594-phalloidin (red).

SUPV3L1 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86706

Clone No.250855G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86706 targets SUPV3L1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3542

  Product name: Recombinant human SUPV3L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 441-786 aa of BC036112

  Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD

• Full Name: suppressor of var1, 3-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 786 aa, 88 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC036112
• Gene Symbol: SUPV3L1
• Gene ID (NCBI): 6832
• RRID: AB_3747857
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IYB8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).