CoraLite® Plus 488-conjugated SYNPO2L Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-83532-3

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SYNPO2L antibody (CL488-83532-3, Clone: 240523F12 ) at dilution of 1:200.

SYNPO2L Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-83532-3

Clone No.240523F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

240523F12, Synaptopodin 2 like protein, Synaptopodin 2-like protein

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83532-3 targets SYNPO2L in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34027

  Product name: Recombinant human SYNPO2L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 35-389 aa of BC142635

  Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR

• Full Name: synaptopodin 2-like
• Calculated Molecular Weight: 977 aa, 102 kDa
• GenBank Accession Number: BC142635
• Gene Symbol: SYNPO2L
• Gene ID (NCBI): 79933
• RRID: AB_3673267
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H987
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).