Synaptophysin Monoclonal antibody
Synaptophysin Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
5B3F2
Cat no : 60191-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, human brain tissue, pig brain tissue, SH-SY5Y cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 9 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 60191-1-Ig is Synaptophysin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Synaptophysin fusion protein Ag11803 |
| Full Name | synaptophysin |
| Calculated Molecular Weight | 313 aa, 34 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC064550 |
| Gene Symbol | SYP |
| Gene ID (NCBI) | 6855 |
| RRID | AB_10915965 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
| IHC protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
| IF protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Behav Immun l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation. | ||
Nat Protoc Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology. | ||
Aging Cell Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. | ||
Cell Death Dis Phosphorylation of Parkin at serine 131 by p38 MAPK promotes mitochondrial dysfunction and neuronal death in mutant A53T α-synuclein model of Parkinson's disease. | ||
FASEB J Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy. | ||
Chemosphere Arsenic exposure via drinking water during pregnancy and lactation induces autism-like behaviors in male offspring mice. |
