SYTL5 Recombinant antibody

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  WB analysis of U-251 using 85108-5-RR

  U-251 cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SK-N-SH using 85108-5-RR

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 85108-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 85108-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85108-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85108-5-RR against Human SYTL5 were performed. The affinity constant is below 1 pM.

SYTL5 Uni-rAb^TM Recombinant Antibody for WB, IF-P, ELISA

Cat No. 85108-5-RR

Clone No.242838D12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, ELISA

Synonyms

Synaptotagmin-like protein 5, Synaptotagmin like protein 5, synaptotagmin like 5, SLP5, 242838D12

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Tested Applications

• Positive WB detected in: SK-N-SH cells, U-251 cells
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85108-5-RR targets SYTL5 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SYTL5 fusion protein Ag21698
• Full Name: synaptotagmin-like 5
• Calculated Molecular Weight: 730 aa, 81 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC131585
• Gene Symbol: SYTL5
• Gene ID (NCBI): 94122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).