Septin 2 Monoclonal antibody, PBS Only (Capture)

• 

  WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

  WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60075-1-Ig (same clone as 60075-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60075-1-Ig (same clone as 60075-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60075-1-Ig (same clone as 60075-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE cells using 60075-1-Ig (same clone as 60075-1-PBS)

  IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50982-1

  Cytometric bead array standard curve of MP50982-1, SEPT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60075-1-PBS. Detection antibody: 60075-2-PBS. Standard:Ag1946. Range: 0.098-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 60075-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60075-1-Ig against Human SEPT2 were performed. The Affinity Constant is 1.23 nM.

• Featured Product
• KD/KO Validated

Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 60075-1-PBS

Clone No.2F3B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5

---

Product Information

60075-1-PBS targets Septin 2 as part of a matched antibody pair:

MP50982-1: 60075-1-PBS capture and 60075-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1946

  Product name: Recombinant human SEPT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-361 aa of BC014455

  Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV

• Full Name: septin 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC014455
• Gene Symbol: Septin 2
• Gene ID (NCBI): 4735
• RRID: AB_2187016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15019
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)