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  • KD/KO Validated

Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2F3B2

Cat no : 60075-1-Ig

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, SEPT2, SEPT2/septin 2, Septin 2, septin2



Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells
Positive IHC detected inhuman renal cell carcinoma tissue, human brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60075-1-Ig targets Septin 2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 2 fusion protein Ag1946
Full Name septin 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC014455
Gene Symbol Septin 2
Gene ID (NCBI) 4735
RRIDAB_2187016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

Protocols

Product Specific Protocols
WB protocol for Septin 2 antibody 60075-1-IgDownload protocol
IHC protocol for Septin 2 antibody 60075-1-IgDownload protocol
IF protocol for Septin 2 antibody 60075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
humanWB

Cell Res

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Authors - Lei Wang
humanWB

J Cell Biol

Septins suppress the release of vaccinia virus from infected cells.

Authors - Julia Pfanzelter
mouseWB,IF

Cell Rep

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Authors - Fernando Calvo
humanCoIP

iScience

Contribution of septins to human platelet structure and function.

Authors - Oleg V Kim
humanWB,IF

Cytoskeleton (Hoboken)

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Authors - Damián Lobato-Márquez
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amaury (Verified Customer) (03-29-2022)

I performed immunofluorescence on K562. It gave me very nice immunolabelling. I used ice-cold methanol to permeabilize the cells.

  • Applications: Immunofluorescence
  • Cell Tissue Type: K562
Septin 2 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:60075-1-Ig)
FH

Stephen (Verified Customer) (01-31-2019)

works fine for WB in HeLa cells 10ug of protein lysate loaded onto 10% NuPAGE gel run I in 1XMOPS buffer blot transferred onto nitrocelluloseAntibody used at 1:2000 incubated overnight at 4 degrees t in 5% milk PBST solution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
Septin 2 Antibody Western Blot, validation (1:2000 dilution) in HeLa (Cat no:60075-1-Ig)