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Septin 2 Monoclonal antibody
Septin 2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 60075-1-Ig
Validation Data Gallery
|Positive WB detected in||LNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells|
|Positive IHC detected in||human renal cell carcinoma tissue, human brain tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE cells, K-562 cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:2000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60075-1-Ig targets Septin 2 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Septin 2 fusion protein Ag1946|
|Full Name||septin 2|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||42-45 kDa|
|GenBank accession number||BC014455|
|Gene ID (NCBI)||4735|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)
A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.
J Cell Biol
Septins suppress the release of vaccinia virus from infected cells.
Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.
Contribution of septins to human platelet structure and function.
A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amaury (Verified Customer) (03-29-2022)
I performed immunofluorescence on K562. It gave me very nice immunolabelling. I used ice-cold methanol to permeabilize the cells.
Stephen (Verified Customer) (01-31-2019)
works fine for WB in HeLa cells 10ug of protein lysate loaded onto 10% NuPAGE gel run I in 1XMOPS buffer blot transferred onto nitrocelluloseAntibody used at 1:2000 incubated overnight at 4 degrees t in 5% milk PBST solution.