Tested Applications
| Positive WB detected in | LNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells, hTERT-RPE cells |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60075-1-Ig targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1946 Product name: Recombinant human SEPT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC014455 Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV Predict reactive species |
| Full Name | septin 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC014455 |
| Gene Symbol | Septin 2 |
| Gene ID (NCBI) | 4735 |
| RRID | AB_2187016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| IF protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| IHC protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| WB protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Septin-2 antibody (Cat# 60075-1-Ig) has 18 citations published in journals including Nature Genetics, Cell Research, Neuron, Cell Reports, Journal of Cell Biology, Advanced Science, Cell Chemical Biology, Blood Advances, Molecular & Cellular Proteomics, iScience, European Journal of Cell Biology, Journal of Cell Science, Cytoskeleton, Small GTPases, Journal of Advanced Research, and bioRxiv. Research fields cover ciliogenesis, cancer biology, viral infections, thrombosis, pyroptosis, platelet biology, Huntington's disease, cystic fibrosis, apoptosis, and plasma membrane repair.
| Species | Application | Title |
|---|---|---|
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. | ||
J Adv Res Pharmacological inhibition of Septins with Forchlorfenuron attenuates thrombus formation in experimental thrombotic mice models with modulating multiple signaling pathways in platelets | ||
Neuron Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.
| ||
Adv Sci (Weinh) Orai1 is an Entotic Ca2+ Channel for Non-Apoptotic Cell Death, Entosis in Cancer Development | ||
Cell Chem Biol Septins promote macrophage pyroptosis by regulating gasdermin D cleavage and ninjurin-1-mediated plasma membrane rupture |
Reviews
What customers say
All three reviews are positive, with ratings of 4–5 stars. Two users tested the antibody for Western Blot (in murine macrophages and HeLa cells) and reported reliable results at dilutions of 1:50,000 and 1:2,000 with overnight incubation at 4 °C. One user performed immunofluorescence on K562 cells and described the labeling as "very nice," using ice-cold methanol for permeabilization. Overall, reviewers found the antibody to work well across both WB and IF applications with clear, specific signals.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Patrick (Verified Customer) (12-15-2025) | Works well with a 1:50,000 dilution in TBS + 5% BSA and overnight incubation at 4 °C.
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Amaury (Verified Customer) (03-29-2022) | I performed immunofluorescence on K562. It gave me very nice immunolabelling. I used ice-cold methanol to permeabilize the cells.
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Stephen (Verified Customer) (01-31-2019) | works fine for WB in HeLa cells 10ug of protein lysate loaded onto 10% NuPAGE gel run I in 1XMOPS buffer blot transferred onto nitrocelluloseAntibody used at 1:2000 incubated overnight at 4 degrees t in 5% milk PBST solution.
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