Serotonin transporter Polyclonal antibody, PBS Only

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• Serotonin transporter Polyclonal antibody, PBS Only (19559-1-PBS)

• 

  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Serotonin transporter Polyclonal Antibody for WB, IF-P, Indirect ELISA

Cat No. 19559-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT

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Product Information

19559-1-PBS targets Serotonin transporter in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa and 120 kDa
• GenBank Accession Number: NM_001045
• Gene Symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• RRID: AB_2878590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31645
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 120 kDa bands in SDS-PAGE. The 120 kDa band corresponds to the glycosylation form of SERT (PMID: 11311448).