Serpin F1/PEDF Recombinant antibody

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  WB analysis of HepG2 using 84685-3-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 84685-3-RR

  C2C12 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 84685-3-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Serpin F1/PEDF Recombinant antibody (84685-3-RR, Clone:242127F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84685-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84685-3-RR against Human Serpin F1/PEDF were performed. The affinity constant is 1.08 nM.

Serpin F1/PEDF Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84685-3-RR

Clone No.242127F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

PEDF, PIG35, 242127F9, Cell proliferation-inducing gene 35 protein, EPC 1

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Tested Applications

• Positive WB detected in: HepG2 cells, C2C12 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84685-3-RR targets Serpin F1/PEDF in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Calculated Molecular Weight: 46kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_002615.7
• Gene Symbol: PEDF
• Gene ID (NCBI): 5176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P36955
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.